People

John Lloyd Associate Chartered Patent Attorney European Patent Attorney European Design Attorney Office Location: Southampton

Specialist Fields

Involved in all types of biotechnology subject matter, particularly microbiology and molecular biology, diagnostics and therapeutics, assay methods, gene sequences and their applications, crystal structures and food technology.

Technical Background

BSc (Hons) in Microbiology (King’s College, University of London).

PhD in Molecular Biology (University of Warwick). PhD research field: Heterologous expression and site directed mutagenesis of soluble methane monooxygenase. Particular emphasis on protein engineering.

One year post-doctoral research experience at the University of Oxford working on protein crystallisation.

Two years experience in the biotechnology industry developing novel nucleic acid amplification and detection technologies.

Professional Experience

Obtained the Certificate in Intellectual Property Law (Queen Mary College, University of London) in 2002.

Clients include individual inventors, universities, international attorneys, spin-out companies and international corporations.

Selected Publications

• X-ray crystal structure of Escherichia coli Taurine/a-ketoglutarate dioxygenase complexed to ferrous iron and substrate(s). Biochemistry, (2002) 41, 5185-5192.
• Specific detection of DNA and RNA targets using a novel isothermal nucleic acid amplification assay based on the formation of a three-way junction structure. Nucleic Acids Research, (2001) 29 e54.
• Homologous expression of soluble methane monooxygenase genes in Methylosinus trichosporium OB3b. Microbiology. 1999 Feb;145 ( Pt 2):461-70.
• Heterologous expression of soluble methane monooxygenase genes in methanotrophs containing only particulate methane monooxygenase. Archives of Microbiology (1999) 171(6), 364-70.
• Heterologous expression of alkene monooxygenase from Rhodococcus rhodochrous B-276. European Journal of Biochemistry (1999) 260(2), 446-52.
• Inactivation of the Regulatory Protein B of Soluble Methane Monooxygenase from Methylococcus capsulatus (Bath) by Proteolysis can be Overcome by a Gly to Gln Modification. European Journal of Biochemistry (1999) 248, 72-79.
• WO 01/09377 and WO 01/09376.

Society Membership

Fellow of the Chartered Institute of Patent Attorneys.